Next Generation Sequencing Services (NGS)

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NIMGenetics offers next-generation sequencing services using platforms with different abilities, with the aim of covering all types of projects, both those that require a high sequencing capacity (NovaSeq 6000) and a moderate one (MiniSeq). 

Sequencing platforms like NovaSeq 6000 provide users with the performance, speed and flexibility to complete projects more efficiently. Availability of multiple types of flow cells, possibility of loading libraries in individual lanes and diverse combinations of read length, allowing adaptation to almost any study need. 

The main NGS sequencing services that NIMGenetics offers are:

GENONIM®, Whole-Genome Sequencing (WGS)

GenoNIM® is our Whole Genome Sequencing (WGS) service, a comprehensive tool available to clinicians and researchers that allows the joint identification of changes in the genomic and mitochondrial DNA sequence, referring to intronic and exonic variants, changes in copy number (CNVs), structural variants (such as rearrangements) or expansions.

Whole Genome Sequencing (WGS) is the most comprehensive tool for tackling highly complex projects, identifying a broad spectrum of biomarkers in DNA, such as:

  • Single Nucleotide Polymorphisms (SNPs)
  • Insertions and deletions (Indels)
  • Structural variants (SV)
  • Copy Number Variants (CNVs)

Genome sequencing based on total DNA fragmentation allows us to analyze regions that, due to their high complexity, are not accessible with capture systems used to prepare exome libraries or panels, revealing regions that cannot be analyzed using other technologies.

While this method of sequencing is commonly associated with human genomes, the scalable and flexible nature of NGS makes it equally useful for sequencing any species, animal, plant, or microbial.

Whole-Exome Sequencing (WES)

Human Whole Exome Sequencing (WES) is a powerful and affordable tool that enables researchers to identify variants contained in the coding region that answer to their hypotheses, without being limited to a specific gene capture.

Likewise, the study of WES applied to populations allows to carry out association studies in the investigation of diseases of complex inheritance. More commonly, it is used to identify variants that originate diverse pathologies with genetic origin.

Transcriptome Sequencing (RNA-Seq, WTS)

RNA-Seq is the first tool available for detecting the presence of RNA and its expression level by means of NGS. Additionally, it enables the detection of new genes structures, alternative splicing isoforms, gene fusions, SNPs, Indels and the specific expression of alleles.

DNA-encoded chemical libraries

DNA-encoded chemical libraries (DEL) is a technology for the synthesis and high-scale detection of collections of small molecule compounds, the objective of which is to accelerate drug screening. At NIMGenetics we offer the service of preparing libraries compatible with sequencing on the NovaSeq 6000, as well as such as data analysis.

Confirm CRISPR edits and off-target analysis with NGS

NGS is the only assay that provides both qualitative and quantitative information at high resolution across the full range of modifications, meets the needs of any throughput, and can be used to monitor off-target effects. NGS-based targeted sequencing provides a cost-effective solution for confirming CRISPR-induced edits and analyze off-target sites previously predicted in silico.

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